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  • biomedres
    18.05.2021 - 1 hour ago

    Journals on Regenerative Diseases - BJSTR Journal

    Physical Activity and Academic Performance among Adolescents – An impact study using Active Science Mobile Application by  Andres Lopez* in  Biomedical Journal of Scientific & Technical Research https://biomedres.us/fulltexts/BJSTR.MS.ID.002169.php Purpose: Obesity has been a significant problem in the United States and the availability for adolescent to participate in physical activity is limited by the reduction in physical education programs. In this study, we will evaluate the effectiveness of Active Science on academic performance and (PA) within a Physical Education (PE) program.Methods: Twenty-five 6th grade students 10 years of age were recruited to participate in the intervention. Baseline activity levels were collected on all participants utilizing 3 days of PA. Prior to intervention participants took a pre-and post-assessments that measured attitude and science knowledge. The 3-week intervention included use of the Active Science mobile application. Participants wore accelerometers throughout the intervention and they performed PA within a PE program. At the end of the session participants imputed accelerometer data into AS app and completed science questions that were based of the Massachusetts STEM.Data Analysis: Paired t-test will be performed to measure difference among groupsResults: There were improvements in average steps for both groups combined baseline steps were 966 and accumulated average was 1438. There was no significant finding among groups in PA. Improvements in science knowledge and attitudes were found for both groups (Group 1, 7% and 6% increase; Group 2, 22% and 14%) but there were no significant findings.Conclusion: Although, the aforementioned research did not have significant data it is a great leading step to improve and validate the effectiveness of PE and its effectiveness on academic performance. For more articles on Journals on Regenerative Diseases please click here bjstr Follow on Twitter : https://twitter.com/Biomedres01 Follow on Blogger : https://biomedres01.blogspot.com/ Like Our Pins On : https://www.pinterest.com/biomedres/

    #Journal of Biomedical Research and Review #Biomedical Research Articles #Biomedical Science Articles #Journal of Scientific and Technical Research #American medical journal
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  • biomedres
    18.05.2021 - 1 hour ago

    Journals on Vaccination - BJSTR Journal

    Method for Selection of Laboratories in Need of Infrastructure Improvement to Meet HIV Viral Load and Early Infant Diagnosis Unmet Testing Needs in Kenya by  Leonard Kingwara* in  Biomedical Journal of Scientific & Technical Research https://biomedres.us/fulltexts/BJSTR.MS.ID.002168.php Introduction: Laboratory infrastructure remains an obstacle for meeting ISO 15189 requirements to assure functional quality management system essentials and competency to generate accurate results. With limited resources, it is challenging to select which facilities to upgrade from among many of similar needs. While common variables in laboratory systems are known, a model that uses these variables to identify priority laboratories for infrastructure upgrade in most resource limited setting is missing.Methods: A quantitative and qualitative based questionnaire was used to collect specific indicators. Principal Component Analysis (PCA) type of factor analysis was then used to generate weights for each indicator. Composite indices were then obtained and used to group laboratories into four ranked clusters.Results: Four clusters were generated in ranked order where the poorest performing facilities n=4, 21.1% were classified in cluster one. Of the 29 indicators the composite range score for the first five was 0.083-0.142. Absence of fire and smoke detectors had the least weight at -0.13. Among the 20 laboratories 9 had a negative factor weight.Discussion: PCA method provides an opportunity to apply quantitative methods for generating weights that can be applied to select laboratories for infrastructure improvement. For more articles on Journals on Vaccination please click here bjstr Follow on Twitter : https://twitter.com/Biomedres01 Follow on Blogger : https://biomedres01.blogspot.com/ Like Our Pins On : https://www.pinterest.com/biomedres/

    #Open Access Medical Journal #Biomedical Open Access Journals #Biomedical Journal Articles #Biomedical Journal Impact Factor #Scientific Research Articles on Biomedical
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  • ankitabelsare
    18.05.2021 - 3 hours ago

    Biomedical Textiles Market Share, Analysis, Size, Share, Growth, Trends, and Forecast 2020 – 2027

    The global Biomedical Textiles Market Share, is spanned across five regions namely Asia Pacific, North America, Europe, Latin America, and the Middle East and Africa. 

    Asia Pacific was the largest market for biomedical textiles in 2017 and is expected to exhibit exponential growth over the forecast period. Growing healthcare industry due to expanding population, rising cases of chronic diseases on account of lifestyle changes, growing foreign investments on account of FDI campaign & relaxed investment norms are likely to boost demand of biomedical textiles during the forecast period. Furthermore, various government initiatives particularly in India such as Air Dispensary, The Intensified Mission Indradhanush (IMI), National Nutrition Mission (NNM), LaQshya, and continuation of National Health Mission are also expected to be contributing to the growth of the regional Biomedical Textiles Market Share, over the forecast period.

    Get Free Sample@ https://www.marketresearchfuture.com/sample_request/6079

    North America and Europe market is relatively mature due to established healthcare facilities and availability of sophisticated products and is expected to provide limited opportunities to the Biomedical Textiles Market Share, over the forecast period.

    The Middle East & Africa is likely to witness rising growth during the forecast period.  GCC countries are expected to witness robust growth during the forecast period owing to various government initiatives such as investments to upgrade hospitals and clinics, build new facilities, and enforce mandatory health insurance schemes in light of local economic, demographic and epidemiological transitions. High rates of illness and mortality caused by chronic diseases due to lifestyle changes are the factor responsible for these government initiatives.

    After the recent economic downturn, Latin America is expected to grow at a moderate CAGR over the forecast period. However, the region is witnessing growing imports of medical equipment, which can be directly linked to the rise in the demand for healthcare facilities on account of rising government initiatives to increase access to healthcare, virtual doctors scheme, and rapidly aging population among others.

    Competitive Analysis

    Some of the key players in the global Biomedical Textiles Market Share, are Secant Group, LLC (U.S.), Confluent Medical Technologies (U.S.), SWICOFIL AG (Switzerland), Johnson & Johnson Services, Inc. (U.S.), Meister & Cie AG (Switzerland), Cardinal Health. (U.S.), Smith & Nephew (UK), Medline Industries, Inc (India), Integra LifeSciences Corporation. (U.S.), PAUL HARTMANN AG (Germany), BSN medical. (Germany), B. Braun Melsungen AG (Germany), 3M (U.S.), Elkem Silicones (Norway), and ATEX TECHNOLOGIES (U.S.).

    Market Segmentation

    The global Biomedical Textiles Market Share, is segmented into fiber type, fabric type, product type, application, and region.

    On the basis of the fiber type, the global Biomedical Textiles Market Share, is segmented into the non-biodegradable fiber and biodegradable fiber.  The non-biodegradable fiber is sub-segmented into polypropylene, polyethylene, polyester, polyamide, polyurethane, polytetrafluoroethylene, and others. Biodegradable fiber is sub-segmented into cotton, viscose rayon, collagen, chitin, and others.

    On the basis of the fabric type, the global Biomedical Textiles Market Share, is segmented into woven, non-woven, knitted & braided fabrics, hollow fabric, and others.

    On the basis of the product type, the global Biomedical Textiles Market Share, is segmented into healthcare & hygiene products, non-implantable products, implantable products, and extracorporeal devices. The healthcare & hygiene products are sub-segmented into surgical clothing, surgical drapes, beddings, sanitary napkins, baby diapers, adult incontinence products, and others. The non-implantable products are sub-segmented into bandages, wound care dressings, plasters, gauze, and others. Implantable products are sub-segmented into soft tissue implants (hernia repair, ligament implant), hard tissue implants (orthopedic implant, dental implant), cardiovascular implants (vascular graft, heart valve), surgical sutures and others. Extracorporeal devices are sub-segmented into artificial kidney, liver, and lungs.

    Based on the applications, the global Biomedical Textiles Market Share, is segmented into ophthalmology, neurology, cardiology, dentistry, orthopedics, general surgery & treatment, and others.

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    https://www.marketresearchfuture.com/reports/biomedical-textiles-market-6079

    #Biomedical Textiles Market
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  • biomedgrid
    18.05.2021 - 5 hours ago

    Biomed Grid | Cell Cycle Dynamics and Endoreplication In the Mosquito Midgut

    Abstract

    The mosquitoes are the major transmissors of vector-borne infections, including virus, protozoans and helminths and, during the interaction it is critical for pathogens to accomplish immune evasion actions whereas the mosquito turn on immune mechanisms for its survival. The mosquito midgut is the organ where initial interactions with pathogens happen, and the insect has mechanisms to repair the tissue and to eliminate the invaders. For these tasks’ mosquitoes use different strategies including the specific DNA replication, amplifying specific genes and without cell division in order to have more DNA templates for transcription that allow, in a secondary infection, the fast production of immune proteins in a process known as priming. The study of these processes is necessary for the understanding of the mechanisms during insect-pathogens interactions, and to design future interventions to block mosquito pathogens transmission that could be environment-friendly and not depending on costly chemicals.

    Introduction

    The mosquito midgut is an essential tissue where pathogens and microorganisms interact. This organ is fundamental to transmit diverse diseases, including the virus causing dengue, zika, chikungunya, and the malaria causing parasite Plasmodium sp. During the interaction with pathogens, the midgut cells react; repairing cell damage and producing immune molecules to fight against invaders. However, we have limited knowledge of the mechanisms behind the response and the cell-damaged repair. The cell cycle is a finely regulated process that manages proliferation and differentiation in cells, which is controlled by a coordinated expression of specific molecules, notably proteins called cyclins. Cyclins are expressed in a particular sequence in specific cell cycle phases and are critical elements for cycle progression [1]. The conventional cell cycle phases include Gap 1 phase (G1), synthesis phase (S), Gap 2 phase (G2), and end in mitosis (M) where the cell splits into two daughter cells. In insects exist specific situations where cells can vary the plan and generate cells with different ploidy and/or DNA content, as in polytenic cells happening in dipteran salivary glands. Interestingly, the machinery that manages the conventional cell cycle is the same, participating in different options of the cell cycle [2-4].

    One of the versions of a specialized cell cycle is the endoreplication. Endoreplication is a variant of the normal replicative cell cycle, in which cells increase their genomic DNA content without division. Endoreplication can enclose different options of the cell cycle; such as endocyles, re-replication, and endomitosis. The first one consists of repeated successions of S –G phases of all genetic material, without cell or nuclei division. In re-replication DNA synthesis is initiated multiple times at individual origins of replication within the same S phase, provoking site-specific replication of a unique sequence. In endomitosis an entry into mitosis occurs, the cells condense the chromosomes but do not dissociate them to daughter cells. Instead, they re-enter a similar phase to G1 and S phase starts again resulting in a multiple nuclei cell. [5]. The typical illustration of endoreplication is the generation of polytenic chromosomes in Drosophila salivary glands, but in many others, insects occur endoreplication. For example, in the beetle Tribolium castaneum larval stages, intestinal stem cells (ISC) conduct endoreplication for adult midgut polyploidic epithelium formation [6]. Also, in the flour moth Ephestia kuehniella, nuclei in Malpighian tubules and silk glands increase in size trough larvae instars. Even, in the last instar, larvae nuclei are polyploid with a high DNA content, provoking a branched nucleus. This polyploidy that results in branched nuclei, could be considered an adaptation because the distance between the nuclear area and the cytoplasmic zone is increased to permit traffic of molecules produced in high quantities [7].

    Here, we discuss the cell cycle in the midgut development and the mechanisms conducting normal cell cycle into endoreplication. We describe endoreplication in the mosquito midgut as a fundamental part of its homeostasis. Finally, we also consider infection as a stressor when tissue undergoes damage and immune stimulation.

    Cell cycle regulation during development

    The regulation of the cell cycle depends on master mechanisms on several levels. Hormones that coordinate the cell cycle between different body parts and more fine mechanisms which act at the cellular/molecular level. Signaling pathways that detect environmental cues, including the hormones and chemicals, master molecules, as cyclins, kinases and phosphatases, that are synthesized and activated/desactivated or destroyed in an strict sequence in order to stop or induce the change from one to next cell cycle phase [8-11] The two main insect hormones, the Juvenile Hormone (JH) and the steroid hormone ecdysone and its active metabolites as 20- hydroxyecdysone (20-E) regulates development and reproductive maturation in insects.

    JH is produced and released by the corpora allata (CA), endocrine glands connected brain [12], and their actions regulate reproduction, behavior, and diapause, between others. In Locusta migratoria, the allatectomy which leads to depletion of CA, inhibits de novo DNA synthesis. Application of Methoprene, a JH analog, restores JH-induced activities, including DNA synthesis and polyploidization in fat body cells. The mechanism involves methoprene- tolerant (Met), a JH receptor that induces transcriptional activation in minichromosome maintenance genes 4 and 7 (Mcm4, Mcm7), both genes involved in genome replication in fat body cells [13]. JH regulates the machinery of the cell cycle to originate polyploid cells supporting the massive synthesis of vitellogenin, one of the main proteins in eggs maturation. Also, another mechanism in fat body cell suggests that JH directs Cdk6 and E2f expression causing polyploid cells [14]. Methoprene is an analog of JH, used for insect control, that is not degraded and maintains its effect for a long time and is useful to evaluate JH actions. High methoprene concentrations avoid cell division in the midgut of larval-pupal transition, and it produces different grades of polyploidy in Ae. aegypti [10].

    On the other hand, ecdysone signaling is an essential mediator in the switch between endocycle and site-specific endoreplication, trough the binding to the ecdysone receptor (EcR). At signaling pathways level, in epithelial follicle cells during oogenesis, Notch signaling is a mechanism that modulates endocycles, switching to site-specific gene amplification. Notch is down regulated while ecdysone signaling is activated and cooperate with Tramtrack (Tkt), a transcription factor that induces endocycle exit and entry in site-specific endoreplication [15]. Moreover, steroid hormone 20-hydroxyecdysone (20E) arrests in G1 phase the mosquito cell line C7-10 from Aedes albopicus and at the same time occurs down-regulation of cyclin A [16].

    A “low-cost” strategy

    Cell proliferation is a demanding process in material and energy terms. During oogenesis in Drosophila melanogaster, a stable nutritional status is important in ovariole to continue with the conventional proliferation program [17]. Also, in oogenesis, after mitotic proliferation occurs a transition to endoreplication that causes an amplification of specific DNA regions in which genes for essential situations are located, and that need to be mass-produced [18]. In nature, many organisms carry out endoreplication to provide a massive production of molecules. This strategy was eventually developed in cell types with high metabolic activity [19]. This hypothesis was proposed in the seeds of plants with endosperm. Endoreplication can enhance metabolic capacity because it occurs an increase in the gene copy numbers of genes related to metabolism, allowing synthesis of high quantities of relevant molecules which will be stored inside seed [20].

    Replication, transcription, and translation have different energetic costs inside a cell. However, replication occurs at least one time, and transcription and translation are constant processes. An analysis in energetic terms from bacteria to eukaryotes indicates that the cost of a gene activity at the DNA, RNA, and protein level decline in relation with cell mass [21]. According to this, in specialized organs or cells, endoreplication would be, and actually it is, a very useful strategy to increase the cell and/or tissue size by polyploidy, generating cells efficient for specialized production during a short time avoiding mitotic cycles and spending less energy.

    Cell cycle dynamics after infections

    The hematophagous insect’s midgut is a monolayer of cells that can distend under the stretching of a large blood volume and absorb the nutrients from the blood meal. In mosquitoes, as in other insects, the midgut cells are differentiated in endocrinal, columnar, and regenerative cells.

    Mosquitoes are the principal bloodfed arthropods responsible for transmitting critical diseases (vector-borne diseases) such as malaria, dengue, Zika, chikungunya, between others. In mosquitoes, there are data suggesting that after infections or immune challenges in specific tissues, including the midgut, cell cycle dynamics change [22], initiating regeneration and/or immunity functions. Cell cycle activation has been documented in Aedes albopictus midgut after chemical and bacterial damage [23]. These stimuli produced an increase in regenerative cells, allowing to the midgut to equilibrate the homeostasis. Besides, after viral infections and oxidative stress, cell proliferation has been tested in Aedes aegypti midgut [24].Evidence suggests that midgut infection is modulated by delayed or immediate activation of ISC to division. This activation is considered a strategy for tissue repair. However, polyploidization and growth by cell fusion also permit tissues to reach homeostasis with lower energy expenditure. It avoids the demand for an energetic process involving proliferation.

    Metazoans maintain tissue size and morphology using cell division as a primary strategy to increase tissue size and repair. Although post-mitotic tissues can undergo compensatory cellular hypertrophy (CCI), consisting in hypertrophic growth without cell division, and this activation depends on the speed-up of endocycles rate, producing a cell with high DNA content and increased size to repair spaces in tissue after damage or apoptosis. Insulin/IGF (insulin- like growth factor)-like signaling pathway is involved in this strategy, intensifying endocycles rendering cells with increased cell size [25]. A hypothesis is that CCI strategy could be displayed during the insect immune response. In Anopheles albimanus tissues with a known relevant immune response and challenged with different microorganism showed a significant DNA synthesis [22,26,27]. This DNA synthesis has been attributed to an endoreplication process because any cell division has been observed. DNA synthesis was evidenced by BrdU incorporation in various tissues after immune challenge with Saccharomyces cerevisiae. Besides, after colchicine treatments an inhibition in BrdU incorporation occurs [26]. These results suggest that DNA synthesis is an active process caused by an immune challenge. Likewise, in Anopheles albimanus DNA synthesis after Plasmodium sp., challenge has been detected using BrdU incorporation [27]. This DNA synthesis is associated to an adaptative immune response, that in insects is known as immunological priming. Priming is defined as the ability to acquire a protective response to a pathogen as a consequence of previous exposure to the same organism. Such response has been probed in many invertebrates’ groups [28]. In An. albimanus, after a second exposure with Plasmodium berghei an increase in HNT, a protein that can regulate Notch pathway in proliferation and differentiation during development in follicular cells, [29] is detected. Similar results in Ae. aegypti after a second exposure to Dengue Virus [30] suggest an essential role of HNT in mosquito immunological priming and after oxidative stress [24]. The mechanism by which immunological priming occurs should be confirmed, but in mosquitoes, DNA synthesis and the expression of molecules related to endoreplication and stress-immunity response as antimicrobial peptides (AMP´s), are exciting results indicating that during the immune response the cell cycle is activated (Figure 1). In addittion, morphologic evidence suggests that after P. falciparum invasion and damage of mosquito midgut epithelium, cells undergo division and differentiation leading to regeneration [31]. However, the mechanism in epithelial repair after cell damage by Plasmodium gallinaceum invasion in Aedes aegypti midgut involves an actin cone that mediates cells displacement without cell division evidence [32].

    Figure 1: DNA synthesis induction in the mosquito midgut, during immunological priming. In an Anopheles midgut columnar cell challenged with Plasmodium berghei HNT activity is activated (1), leading to amplification of DNA specific regions (colored DNA double helix). Among the amplified genes are stress-immunity response molecules and antimicrobial peptides (AMP´s) (site-specific endoreplication) (2). In a second Plasmodium challenge (3), transcription of amplified genes occurs rapidly leading to an increase in expression of immune molecules which respond against Plasmodium. The light-yellow cell adjacent to basal lamina corresponds to a regenerative cell. Endocrine cells were omitted. PM.- Peritrophic matrix. AMPs. - Antimicrobial peptides. HNT. - Hindsight protein.

    Conclusion

    In insects, particularly in dipterans as flyes and mosquitoes, cells needing the massive synthesis of molecules, required during reproduction and other tasks, carry-out the amplification of DNA segments, bearing genes specific to be used as templates for extensive transcription of messengers that will be used to produce useful molecules. This dynamic response is “low-cost” compared with other strategies, including the cell division, which needs a lot of material, energy, and time. There is increasing evidence that in mosquitoes, this strategy can be deployed to face immune challenges, and to prepare the individuals against repetitive pathogens assaults. These actions lead the mosquitoes to a “primed” status, in which the insect will be resistant to specific infections. Although there is a lot of molecules and mechanisms that are unknown in this critical group of insects. This is an exciting field that should be explored; to understand the mosquito-pathogens relations, and to design creative strategies to interfere with disease transmission.

    Read More About this Article: https://biomedgrid.com/fulltext/volume5/cell-cycle-dynamics-and-endoreplication-in-the-mosquito-midgut.000871.php

    For more about: Journals on Biomedical Science :Biomed Grid | Current Issue

    #biomedgrid #American Journal of Biomedical Science & Research #Biomedical science articles #Open access Journals of Biomedical Science
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  • aeide-thea
    18.05.2021 - 9 hours ago
    #identity#trans things #like. she definitely frames Gender Yearning as a ~qualifying~ but interim state #to which biomedical transition is the Next Step #and i'm not sure i want to frame it so directionally #but i think the point about like. the relationship between yr dreams and yr identity is pretty dece
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  • poetic-storm
    17.05.2021 - 16 hours ago

    So my question is, do I want to find a better way to treat mental health issues without the use of drugs, find a new and more effective treatment for cancer, or create better man made environments for astronauts to minimize the longterm effects of space on their bodies?

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  • santoshbvn
    17.05.2021 - 23 hours ago

    COVID hospital: AP Pollution Board develops app for disposing biomedical waste from COVID hospitals

    COVID hospital: AP Pollution Board develops app for disposing biomedical waste from COVID hospitals

    The Andhra Pradesh Pollution Control Board has developed a mobile app for effective management and scientific disposal of biomedical waste from Covid-19 hospitals and quarantine centres. Over 600 notified Covid-19 hospitals in the state are now generating about 38 tonnes of biomedical waste per day and scientific disposal of this has posed the biggest challenge for APPCB, according to its…

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  • chetanare
    17.05.2021 - 1 day ago

    Biomedical Refrigerators Industry Research Report, Growth Trends and Competitive Analysis 2021-2027: Haier, Sanyo (Panasonic), Dometic, Zhongke Meiling, Thermo, AUCMA, Helmer

    Biomedical Refrigerators Industry Research Report, Growth Trends and Competitive Analysis 2021-2027: Haier, Sanyo (Panasonic), Dometic, Zhongke Meiling, Thermo, AUCMA, Helmer

    Overview The global Biomedical Refrigerators market report offers a comprehensive insight into the development policies and plans in addition to manufacturing processes and cost structures. It provides a basic overview of the industry including definitions, classifications, applications and industry chain structure. A professional and in-depth research on the current state global industry, this…

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    #Biomedical Refrigerators #Biomedical Refrigerators industry #Biomedical Refrigerators industry Analysis #Biomedical Refrigerators industry Growth #Biomedical Refrigerators industry Growth Size #Biomedical Refrigerators market #Biomedical Refrigerators market Analysis #Biomedical Refrigerators market Growth #Biomedical Refrigerators market Share #Biomedical Refrigerators market Size #Biomedical Refrigerators market Trends
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  • mumblejournal
    17.05.2021 - 1 day ago

    i was allowed s double sided handwritten “cheatsheet” for my exam today, honestly the exam was alot harder than any of the practice questions and i dont think i did that well :(

    but i was proud of the cheat sheet so idk

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  • onsitewastetechnologies
    17.05.2021 - 1 day ago
    #medical waste disposal #biomedical waste disposal #medical sharps container #medical sharps container disposal #sharps waste bin #medical waste disposal near me #waste disposal solution
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  • healthcare-engineering
    17.05.2021 - 1 day ago

    " Healthcare Engineering▶️ provides you more contemporary healthcare concepts & new biomedical project ideas💡" For instant updates kindly follow our YouTube channel-HEALTHCARE ENGINEERING( Link in the bio 🔗) https://youtube.com/channel/UCdA2b8fkoRQ1Kh6KBYdlqBw & follow @healthcare_engineering #healthcareengineering #healthcareworkers #healthcareprofessionals #healthcareengineers #medicalengineering #medicalengineers #biomedicalengineering #biomedicalengineers #biomechanics #clinicalengineering #clinicalengineers #medicaldevices #biomedicalinstrumentation #biomedicalprojects #projectideas #healthcare_engineering #biomedical #biomed #biology #engineering #bioengineering #didyouknow #didyouknowfacts #instafacts #factsdaily #instagram #instadaily #instagood #instamood #likeforlikes Contact email ID : sam.gastondiaz@gmail.com https://www.instagram.com/p/CO9sqfwjY7i/?igshid=ujwwefdljl0w

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  • jobssydneyaustralia
    16.05.2021 - 1 day ago

    Assistant Product Manager

    About the Role This is a 12 months fixed term maternity cover contract role! As an Assistant Product Manager, you will be working with your manager and alongside the marketing and customer experience team, you will assist with key programs to support the consumer product portfolio across the Sales & Support Group business. Primary responsibilities Assist in the product management of all…

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    #apprentice chef jobs sydney #art director jobs sydney #biomedical science jobs sydney #casual jobs sydney no experience #chef jobs sydney #christian jobs sydney #clinical nurse educator jobs sydney #council childcare jobs sydney #devops jobs sydney #diagnostic radiographer jobs sydney #event manager jobs sydney #gumtree jobs sydney region #healthcare jobs sydney #part time jobs sydney cbd #sales assistant jobs sydney #shop assistant jobs sydney #sports coaching jobs sydney #student engineering jobs sydney #tunnel labourer jobs sydney #work health and safety jobs sydney
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